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Use PCR purification kit to purify restriction digestion

cleanup restriction digestion

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2 replies to this topic

#1 Philosopher

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Posted 28 July 2012 - 07:24 AM

Hi I juste wanted to know if PCR purification kit could be used for purifying restion digestion too ?

Thx by advance

#2 phage434

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Posted 28 July 2012 - 09:50 AM

Yes.

#3 hobglobin

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Posted 28 July 2012 - 10:20 AM

The only issue might be that you lose small DNA fragments (usually around 100 bp and smaller). But in most applications you anyway don't need them...
  
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.





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