Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Use PCR purification kit to purify restriction digestion

cleanup restriction digestion

  • Please log in to reply
2 replies to this topic

#1 Philosopher

Philosopher

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 28 July 2012 - 07:24 AM

Hi I juste wanted to know if PCR purification kit could be used for purifying restion digestion too ?

Thx by advance

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,410 posts
234
Excellent

Posted 28 July 2012 - 09:50 AM

Yes.

#3 hobglobin

hobglobin

    Growing old is mandatory, growing up is optional...

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,519 posts
95
Excellent

Posted 28 July 2012 - 10:20 AM

The only issue might be that you lose small DNA fragments (usually around 100 bp and smaller). But in most applications you anyway don't need them...
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.