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Do you know about reverse pipetting? A survey

pipetting techniques accuracy

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17 replies to this topic

Poll: Reverse pipetting survey (27 member(s) have cast votes)

You and reverse pipetting

  1. Never heard about it (until I read this) (8 votes [29.63%] - View)

    Percentage of vote: 29.63%

  2. I know what it is, but I never used it (3 votes [11.11%] - View)

    Percentage of vote: 11.11%

  3. I use it seldom or for special applications only (foamy, viscous samples) (13 votes [48.15%] - View)

    Percentage of vote: 48.15%

  4. I use it even in non-special circumstances/all the time (3 votes [11.11%] - View)

    Percentage of vote: 11.11%

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#16 Ameya P

Ameya P

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Posted 23 August 2012 - 07:49 AM

use it to aliquot PCR master mix. read it in a pipette instruction manual (geeky, yes we all are)

Guess Adrian and me are the only ones (so far) who do it.... thus the 2 votes :)

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#17 Trof


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Posted 23 August 2012 - 12:18 PM

Well, unless all you do in lab is PCR master mix pipetting, then I would say you only use it for special applications, like the majority ;)

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#18 LabLackey



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Posted 28 August 2012 - 09:51 AM

I've been using this technique to rapidly distribute loading buffer amongst samples. It's quite a bit quicker than standard pipetting!

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