Hi, everyone,
I am looking forward to a methods, which can generate a good cDNA library into plenti6/V5 vector.
Right now, i have a cDNA library in pCMV-SPORT6, which have a attB sequence. I would like to use BP reaction to transfer the cDNA library to pDOnor vector, then transfer again by LR reaction to plenti6/V5 vector.
I would like to know is there anyone once play with cDNA library before? especially in the gateway system, as I would like to findout is there any publications using the two steps stratyge to generate a very nice library?
Best
dysbindin
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