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question about universal and specific primers for miRNA real time pcr

miRNA qPCR

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#1 natgiall

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Posted 25 July 2012 - 12:05 PM

What is the difference between universal and specific primers used in miRNA real time qPCR?
My question basically is about the universal ones. What kind of results will the use of these primers give me?

#2 Trof

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Posted 30 July 2012 - 07:21 AM

cDNA from miRNA is made with adapters, universal primers bind the adapter sequence that is the same for all miRNAs. In qPCR you use it in combination with a forward specific primer to amplify only desired miRNA. AFAIK you can't use just universal primer alone.

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