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How to measure expression the mRNA (quantitative)


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#1 Bassam27

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Posted 25 July 2012 - 02:46 AM

do any one know how to measure expression the mRNA (quantitative) from who blood with out using agros gel electrphorosis ?

bcz i want to use whole blood to measure the level expression of mRNA ? AND Read the protocal of RT PCR BUT I i do not get .
and i know i need to use housekeeping gene as internal control but i do not know if i have to ues taqman or SYBR Green?

#2 doxorubicin

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Posted 26 July 2012 - 03:12 AM

It sounds like you will need help from someone with some experience. Try to find someone in a neighboring lab who understands the basics. SYBR green is a very good option. With this, you can purify PCR products and use these to generate a standard curve that you can use to determine the absolute quantity of each mRNA in each sample. This thread may be helpful: http://www.protocol-...standard-curve/




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