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Liver S9 Microsomes with NADPH and Glucose 6 Phosphate


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#1 net123

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Posted 24 July 2012 - 12:17 AM

Hi, so I am looking at protocol for metabolizing drugs using liver microsomes. I am just wondering what the need for NADPH and glucose 6 phosphate reagents are. I understand that NADPH is necessary for the oxidation of the the substance but what use does glucose 6 phosphate have?

Thanks.

#2 Inbox

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Posted 26 August 2012 - 06:34 AM

You must be meaning NADP+ or NAD+ required for oxidation. NADPH is produced in oxidation like one in beta oxidation.
About glucose-6 phosphate it may acting be energy source. Will you describe your question more clearly?

Edited by prabhubct, 26 August 2012 - 09:48 AM.





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