Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

co-eluted protein with his-tag protein


  • Please log in to reply
1 reply to this topic

#1 alinud

alinud

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 23 July 2012 - 11:51 PM

Hello,
I have recently expressed a protein in BL21, about 31 kDa with Histag (Flexi system, Promega). I tried to purify the protein using MagneHis Protein Purification System (Promega). I find that my elute contains most protein in the host cell. I’ve tried increasing the NaCl conc in the wash buffer, but the problem still exists.
Any suggestion what’s happening? Thanks for help.

#2 Inmost sun

Inmost sun

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 303 posts
60
Excellent

Posted 24 July 2012 - 08:13 AM

did you check if your protein of interest was really overexpressed? If so, was it prominent in the elutions?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.