RT-PCR vs plate reader for pathogen detection
Posted 23 July 2012 - 12:10 PM
If I want to do pathogen detection, and don't care about quantification, what are the advantages of RT-PCR vs using RT-PCR chemistries in a traditional PCR machine, and measuring the result in a plate reader? It seems to me that this combination of equipment would be much cheaper than a qPCR machine, but wondering what I am missing. People always seem to do a gel with end point PCR, so not sure why fluorescent detection is not more common. Thanks for any insight!
Posted 23 July 2012 - 03:39 PM
Incidentally, RT-PCR is reverse transcriptase-PCR, you are talking about real-time PCR or qPCR, just to help you avoid confusion.
Posted 23 July 2012 - 03:58 PM
Posted 24 July 2012 - 02:44 PM