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Dual Crosslinking using EGS

EGS precipitation

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#1 chipy

chipy

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Posted 23 July 2012 - 10:33 AM

Hi there,

I'm trying to double crosslink a sample for TF chip.
I'm using EGS and I'm disolving it in DMSO (freshly prepared 1M stock).
I've being trying to use it at 5 mM in PBS, but as soon as I add it to my samples I see a precipitate.
Don't know how to get rid of it. Should I crosslink it also in DMSO??


THANKS!!




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