Hi,
I need help
how do i check if the primers I have are for mouse with BLAST?
I have no idea how to use the link
0
How to check primer sequences using BLAST and other tools?
Started by nataly, Jul 23 2012 04:30 AM
primer blast
2 replies to this topic
#2
Curtis
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Posted 23 July 2012 - 05:47 AM
there is a drop down menu in the middle of the page you need to change that to mouse. It is on human by default.
#3
pcrman
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Posted 23 July 2012 - 12:48 PM
You can use splicenter to check your primers http://projects.insi...ter/PrimerCheck
To use NCBI blast, go to http://www.ncbi.nlm....K_LOC=BlastHome, In the primer parameters section, type your primer sequences
You can also use UCSC in silico PCR tool here http://genome.ucsc.e...n/hgPcr?db=hg18
To use NCBI blast, go to http://www.ncbi.nlm....K_LOC=BlastHome, In the primer parameters section, type your primer sequences
You can also use UCSC in silico PCR tool here http://genome.ucsc.e...n/hgPcr?db=hg18
Also tagged with one or more of these keywords: primer, blast
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