Sf9 cell suspension cultureInsect cell culture
Posted 22 July 2012 - 04:29 AM
I have been growing Sf9 cells in suspension culture medium using TNM-FH-10% FCS and glass Erlenmeyer flasks.
When I use 125 ml Erlenmeyer flask with 25 mL of culture medium, I observe the formation of a ring of cells on the wall of the flask at the interface between air and culture medium. However, the cell growth and viability sounds good.
When I use 250 ml Erlenmeyer flasks with 50 ml of culture medium, the ring of cells become more prominent and, sometimes, the cells in the ring start to die.
What should I do to prevent death of cells in the ring or the formation of the ring altogether?
Posted 22 July 2012 - 09:11 AM
Posted 22 July 2012 - 01:57 PM
Are Fernbach flasks made of polycarbonate?
Have you ever compared growing Sf9 cells in Fernbach and Erlenmeyer flasks?
Do you prevent the formatrion of the ring of cells by growing Sf9 cells in Fernbach flasks?
Posted 22 July 2012 - 02:11 PM
Posted 23 July 2012 - 04:27 AM
What you say sounds right.
However, don't you think that a flask made with a material with low tendency to adhesion (such as polycarbonate or, for the sake of argument, teflon) would prevent the formation of the ring of cells?
Posted 23 July 2012 - 03:28 PM