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competent cell formation

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#1 siddharthsameer



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Posted 22 July 2012 - 03:41 AM

hello all
I have a question regarding competent cell formation of E.coli TOF 10
1.Can we use ddH2O to dissolve the pellet to form competent cell rather than Using Cacl2. Since I am and in my lab I saw people using ddH2O instead of Cacl2 but I have never found a protocol using water,
Kindly tell me about this..
with regards

#2 bob1


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Posted 22 July 2012 - 02:03 PM

Follow the protocol you have for top10, these things have all be worked out for you! Note that the protocol you are actually using is just a general one for competent cells rather than TOP10 specifically.

Different protocols for different preparations... water is often used for electrocompetent cells.

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