Sorry if this question is rather stupid, but I'm very new to cell culture.
Yesterday I had to split some HEK293T cells for the first time. I trypsinated them, diluted with fresh media, spun them down and resuspended the cells in 1.5mL fresh media.
Then I had to leave the lab unintentionally for my supervisor wanted me to join some stupid lecture. He said I can put the cells on ice during this time, and so I did...
Now the question I have - how long is it possible to store suspended cells on ice before they die or take any damage? I left the lab for about 2 hours, so that's pretty long in my opinion. When I got back the cells were like a dense pellet in the falcon tube. When seeded to new culture flasks they looked okay, but I'm nervous anyway... so do I have to expect a mess on monday or will my cells be fine?!
Edited by 2xzwei, 21 July 2012 - 03:07 AM.