Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How long are cells viable on ice

cell viability ice

  • Please log in to reply
2 replies to this topic

#1 2xzwei

2xzwei

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 33 posts
1
Neutral

Posted 21 July 2012 - 03:06 AM

Hi!
Sorry if this question is rather stupid, but I'm very new to cell culture.
Yesterday I had to split some HEK293T cells for the first time. I trypsinated them, diluted with fresh media, spun them down and resuspended the cells in 1.5mL fresh media.
Then I had to leave the lab unintentionally for my supervisor wanted me to join some stupid lecture. He said I can put the cells on ice during this time, and so I did...

Now the question I have - how long is it possible to store suspended cells on ice before they die or take any damage? I left the lab for about 2 hours, so that's pretty long in my opinion. When I got back the cells were like a dense pellet in the falcon tube. When seeded to new culture flasks they looked okay, but I'm nervous anyway... so do I have to expect a mess on monday or will my cells be fine?!
Thanks!

Edited by 2xzwei, 21 July 2012 - 03:07 AM.


#2 leelee

leelee

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 652 posts
53
Excellent

Posted 21 July 2012 - 04:19 AM

I wouldn't worry, your cells will be fine :)

The pellet you saw is normal, as the cells will settle out of the media over time. And I don't believe that being on ice for that short a period will have any impact on viability, and if there is some impact it will be minimal.

#3 2xzwei

2xzwei

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 33 posts
1
Neutral

Posted 23 July 2012 - 02:38 AM

OK, thanks. Fortunately, my cells looked quite nice today ;)





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.