3 replies to this topic

[Abimbola Badejo]

hello, I'm trying to choose an internal reference gene among a group of 7 "HKG" for a bacterial gene expression study.
I've read some journals and I've also gone through most of the ones here  
http://www.protocol-...rs/page__st__30.

My problem is the experiment itself. I'm really confused about the genes.

1. Do I set up my RT-PCR plate with only one target gene and use all the ref genes in separate experiments, after which I'll collate all my data and load on gNORM or
2. I should use only one target gene as the "reference gene" , then use all the reference gene candidates as the "target genes"?
I'm actually a novice in qPCR and I've been struggling with this for the past 3 weeks.
I also have 6 samples ( 3 environmental conditions on a control and a test strain)

In summary, can someone please explain to me what I have to do, I beg you please...............?

[tea-test]

hi, do you have the old excel-based geNORM software?

I have uploaded the handbook where every step is explained in detail.

Attached Files

•  geNorm kit handbook SYBR.pdf   294.08K   1068 downloads

[Abimbola Badejo]

Wow! Thank you tea-test. The manual is a great help. I will let you know of my progress after digesting the manual.

[coffeebreak]

HI!

I have been looking for the old version of geNorm for ages!! (the one for excel). Does somebody know if it is still possible to get it and how?

Thanks!