Experiment plan for RT-qPCR internal reference searchendogenous referenc
Posted 20 July 2012 - 12:09 AM
I've read some journals and I've also gone through most of the ones here
My problem is the experiment itself. I'm really confused about the genes.
1. Do I set up my RT-PCR plate with only one target gene and use all the ref genes in separate experiments, after which I'll collate all my data and load on gNORM or
2. I should use only one target gene as the "reference gene" , then use all the reference gene candidates as the "target genes"?
I'm actually a novice in qPCR and I've been struggling with this for the past 3 weeks.
I also have 6 samples ( 3 environmental conditions on a control and a test strain)
In summary, can someone please explain to me what I have to do, I beg you please...............?
Posted 20 July 2012 - 02:00 AM
I have uploaded the handbook where every step is explained in detail.
Posted 20 July 2012 - 06:27 PM
Posted 24 July 2012 - 12:35 AM
I have been looking for the old version of geNorm for ages!! (the one for excel). Does somebody know if it is still possible to get it and how?