Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Use dialysis tube to concentrate virus

Dialysis tube virus

  • Please log in to reply
8 replies to this topic

#1 xxcici

xxcici

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 64 posts
0
Neutral

Posted 19 July 2012 - 06:19 PM

Hi, I am trying to use dialysis tube to concentrate my virus. Then today, I used buffer without virus inside the dialysis tube and 10% polyethylene glycol (MW is 35000) solution outside the dialysis tube for the experiment. The cutoff molecular weight of the dialysis tube is 3500. After two hours, I did not notice the decrease of buffer inside the tube. I searched the poster in this website and find one posted in 2006 saying by someone that this method is very fast which may take less than 1 hour. So, I want to ask if somebody know what happened to my experiment. Thank a lot!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,666 posts
396
Excellent

Posted 20 July 2012 - 12:06 AM

What concentration buffer did you use inside and outside?

#3 xxcici

xxcici

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 64 posts
0
Neutral

Posted 20 July 2012 - 05:20 AM

glycine buffer with pH of 9.5 and 0.3 M NaCl.

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,666 posts
396
Excellent

Posted 20 July 2012 - 04:35 PM

Would you notice an less than 10% change in volume?

#5 xxcici

xxcici

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 64 posts
0
Neutral

Posted 21 July 2012 - 08:50 AM

Yes, it needs time to make the volume reduced. But why somebody ever said that the volume could be reduced super fast. Here is the link: http://www.protocol-...sts/20733.html.

#6 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,442 posts
242
Excellent

Posted 21 July 2012 - 02:11 PM

Was it a typo that your MW of PEG was 35,000? I have 3500 and 8000 on my shelf, but not 35,000. If it was really 3500, then your dialysis membrane will be letting it through.

#7 xxcici

xxcici

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 64 posts
0
Neutral

Posted 23 July 2012 - 06:31 AM

Yes, I am using PEG with MW of 35 000, not 3500 and 8000.

#8 jakester

jakester

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 23 July 2012 - 12:37 PM

Why not try spin ultrafiltration, i.e. with an Amicon or Spin-X 100KD MWCO filter? Bam, you're done.

#9 xxcici

xxcici

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 64 posts
0
Neutral

Posted 23 July 2012 - 01:39 PM

Hi Jakester, thank you very much for your suggestions. Could you provide me with more information about the spin ultrafiltration? I do not know where I can get that stuff and how it looks like, as well as how to use it. Thanks again.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.