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Where can I find the extinction coefficient of glutathione (GSH)?


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#1 nataly

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Posted 18 July 2012 - 10:43 PM

I've tried to quantify the GSH level in liver tissue. my calibration curve didn't work so i'm trying to calcuate with epsilon instead.
any ideas?

#2 bob1

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Posted 19 July 2012 - 01:30 PM

Are you assaying the glutathione directly or using an indirect assay (e.g. compound that reduces glutathione, measure colour change of said compound)?

#3 nataly

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Posted 04 August 2012 - 02:53 PM

I'm using nature protocol: http://www.nature.co...t.2006.378.html

#4 bob1

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Posted 05 August 2012 - 02:06 PM

So you are actually measuring the change in a compound that is not glutathione - are you sure that you shouldn't be looking for the extinction coefficient for DTNB or TNB?




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