I've tried to quantify the GSH level in liver tissue. my calibration curve didn't work so i'm trying to calcuate with epsilon instead.
any ideas?
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Where can I find the extinction coefficient of glutathione (GSH)?
Started by nataly, Jul 18 2012 10:43 PM
3 replies to this topic
#2
bob1
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Thelymitra pulchella
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Posted 19 July 2012 - 01:30 PM
Are you assaying the glutathione directly or using an indirect assay (e.g. compound that reduces glutathione, measure colour change of said compound)?
#3
nataly
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#4
bob1
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Thelymitra pulchella
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Posted 05 August 2012 - 02:06 PM
So you are actually measuring the change in a compound that is not glutathione - are you sure that you shouldn't be looking for the extinction coefficient for DTNB or TNB?
