Drop plate technique
Posted 18 July 2012 - 05:14 AM
For example, I prepare dilution like this.
Change pipette tip when transferring mixtures to the next dilution in each step.
However, I'd like to know that Can I use the same pipette tip when plating out from high to low dilutions or not (from 10^-5 to 10^-1)? The number of bacteria will be the real number or not?
Thanks for answer.
Posted 18 July 2012 - 01:58 PM
Posted 19 July 2012 - 04:48 AM
From not diluted to diluted: change..
Its pretty easy to understand.
If you go from 10^-6 cells/ml to 10 cells/ml then why change a tip? You are not going to "add" cells in the 10 cells/ml
Other way around....
Same goes for chemical dilutions etc..
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.