Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Weird qPCR curve - fluorescence decline after

Started by mind-boggling, Jul 17 2012 10:37 PM

qPCR weird curve

Please log in to reply

No replies to this topic

#1 mind-boggling

member

Members
1 posts

0
Neutral

Posted 17 July 2012 - 10:37 PM

Hi

I have a weird amplication plot from a SYBR qPCR run (as attached). The plot declines after about 22 cycles.

I have tried running simultaneously, with some other samples and these other samples turned out to be fine. Mastermix and plates are all the same.
The only thing that is changed is the cDNA sample. Do you think it is possible that the cDNA sample is contaminated? However the melting curve gives a nice distinct peak.

Thanks for any advice!

Attached Thumbnails

Back to PCR, RT-PCR and Real-Time PCR · Next Unread Topic →

Also tagged with one or more of these keywords: qPCR, weird curve

Protocols and Techniques Forums → Tissue and Cell Culture → Housekeeper for HEK cells? Started by Guest_Stephp_* , 17 May 2013 HEK cells, house keeper, PCR and 1 more...	1 reply 68 views	Curtis 18 May 2013
Protocols and Techniques Forums → PCR, RT-PCR and Real-Time PCR → FAM and VIC S-curves Started by Guest_squallweathered_* , 23 Apr 2013 qPCR, FAM, VIC, DNA, RNA	0 replies 165 views	squallweathered 23 Apr 2013
Protocols and Techniques Forums → siRNA, microRNA and RNAi → miRNA quantification Started by Guest_dude3005_* , 18 Mar 2013 qPCR, reverse transcription and 1 more...	3 replies 328 views	imoani 26 Mar 2013
Protocols and Techniques Forums → PCR, RT-PCR and Real-Time PCR → Confused about normalization Started by Guest_SillySaru_* , 14 Mar 2013 qpcr, normalization, pfaffl	2 replies 260 views	memari 20 Mar 2013
Protocols and Techniques Forums → Molecular Biology → qPCR detection Started by Guest_wdk_* , 28 Feb 2013 qPCR, RT-PCR	2 replies 133 views	wdk 01 Mar 2013