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Can I use MOPS buffer instead of PIPES in bacteria cells?

PIPES MOPS molecular cloning

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#1 csalat

csalat

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Posted 17 July 2012 - 05:19 AM

I am following the Current Protocols in Molecular Biology to make competent cells with the CaCl2 method. To prepare the CaCl2 solution they use PIPES as a pH buffer. Do someone know if I could use MOPS instead of PIPES for this application?

Thanks!!





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