Hi there,as a newer,there is a question buzz me.It is why there is a plasmid clone of the nested PCR production?Why not directly PCR clone there?Please help me,I will appreciate your kindness!!!
Hope for your answers!
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pcrman
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Excellent
Excellent
Posted 20 July 2012 - 05:17 PM
you can directly sequence your pcr products of course. Sequencing after cloning gives your cleaner and more accurate results.
