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RNA caculation!!


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#1 Ghenada

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Posted 16 July 2012 - 09:37 PM

hi,
i have a simple question but i just want to double check please.
when calculating the RNA con. i am getting an RNA con. on the nano drop 68 ng/ul, the volume i have of RNA is 60ul, what this mean when i calculate RNA in ug?

and another question, how much do i need of RNA when i run RT-PCR??? this is my first time.
thank u.

#2 Astilius

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Posted 16 July 2012 - 11:51 PM

Well, you have 68ng/ul.
That is 68ng of RNA in every ul
You have 60ul of this.
So, simply multiply 60 X 68 = 4080ng RNA

As for how much you need for RT-PCR -- what does your protocol say?
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#3 Ghenada

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Posted 17 July 2012 - 12:00 AM

thank u, yes that what i have caudated.




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