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Sequencing of the PCR production in BSP

DNA methylation

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#1 henry_cheng

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Posted 16 July 2012 - 10:32 AM

Hi there,as a newer,I want to konw how many ways to sequence of the PCR production in BSP? What's diffirences between the methylation/unmethylation primer and inner/outer primer?I will apprecate your kindness.....

#2 pcrman

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Posted 16 July 2012 - 03:10 PM

there are only two ways of sequencing your BSP pcr products: using either forward or reverse primers used in final PCR reaction. Reverse primer usually gives better results than forward.





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