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Primary Dermal Fibroblast isolation

Senescence

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#1 alextam

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Posted 16 July 2012 - 01:59 AM

Hi guys,

Lately when I isolate mice DF, the yield of cells is low and cells won't proliferate. The fibroblasts become large and striated (senescence).

I use this protocol: http://www.mechamlab..._Skin_Cells.pdf

However at the end I do an extra spin step to remove the collagenase.

Do you guys have any solution or ideas?

Thanks!

#2 alextam

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Posted 18 July 2012 - 01:36 AM

However right now I am not sure whether these cells are going senescent since these cells are only Passage 0-1. So I am thinking that these cells might be differentiating to myofibroblasts...

Anyone has suggestions on this or how to prevent this?





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