My question is, does this step seperate the antibody from chromatin? In another word, is the antibody still bound with chromatin in the supernatant or it is discarded with the beads?
I'm planning to do a pilot experiment for re-ChIP. Unfortunately two antibodies are both anti-mouse. In this case it is necessary to make sure no remaining 1st antibody after the 1st round elution. Not sure this will work. Anyone has any suggestions?
Edited by doubtdoubt, 15 July 2012 - 07:07 PM.