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Tricine SDS-PAGE - Protein and Proteomics - BioForum

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Tricine SDS-PAGE


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3 replies to this topic

#1 le_duy211

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Posted 14 July 2012 - 05:45 PM

Is there anybody know why tricine sds-page can separate the low molecular weight proteins better than glycine sds-page ???

#2 mdfenko

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Posted 16 July 2012 - 05:34 AM

this should help:

Attached Files


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genius does what it must
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#3 le_duy211

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Posted 22 July 2012 - 07:25 PM

Thank you so much. I've read the file attached but I still don't know the role of tricine in this method and what is the difference between using tricine and glycine.

#4 mdfenko

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Posted 23 July 2012 - 07:34 AM

tricine migrates more slowly than glycine. this allows for improved separation of low molecular weight proteins and peptides at lower acrylamide concentrations than with glycine.

i don't have an electronic copy of the paper in which this is described but here is a link to it: shagger and von jagow.
talent does what it can
genius does what it must
i do what i get paid to do




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