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is outdated blood suitable for PBMC isolation?

PBMC isolation

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#1 joy123

joy123

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Posted 13 July 2012 - 07:56 AM

Hi, I want to extract PBMC from blood for IFN-gamma ELISPOT assay.

We have outdated in blood center of our hospital. I wonder if these blood is suitable for PBMC isolation?

Or I must use fresh blood to get PBMC?

Thanks!

#2 Curtis

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Posted 15 July 2012 - 06:15 PM

I think you can use it. People sometimes get them from blood banks




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