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How to quantify/visualize cell surface proteins without FACS

Cell surface marker Flow cytometry microplate readers

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#1 mdarley



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Posted 13 July 2012 - 07:18 AM

Happy Friday!

But I have a bit of a conundrum...

I have recently graduated and taken a new job starting up a tissue culture/efficacy testing lab at a personal care company. I've been trained as an immunologist so my go to has been FACS but I do not have that equipment in my new lab.

I was to look at cell adhesion molecules but need a new way to do it. I have a BioTek microplate reader that can do all kinds of things. I'm thinking I could possibly prep my cells as if I was doing a FACS experiment, plate them in a 96 well plate, and read the fluorescence on the microplate reader.

Does anyone have any suggestions on the above idea or other types of assays I could do?



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