Expression and Lysozyme
Posted 13 July 2012 - 01:15 AM
My problem is that my desired protein to be expressed is ~14 KD and when I add lysozyme as part of the expression protocol (about 10mg) I get an inactive protein which I think is just the remaining lysozyme. I use expression by sonication using BL21 cells.
Is there any way around this? Is using lysoyme necessary for expression and protein extraction, or is there any alternative to lysozyme that is not 14kd?
Thank for your help in advance.
Posted 25 July 2012 - 05:40 AM
Posted 14 March 2013 - 12:57 PM
I've only used the FastBreak lysis reagent from Promega so that is the only one I can recommend from experience.
Posted 15 March 2013 - 07:39 AM