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Is the genomic DNA similar among different cell lines?


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4 replies to this topic

#1 gyma

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Posted 12 July 2012 - 10:47 PM

Hi everyone, I tried to amplify a promoter region of my interested gene from genomic DNA of a cell line. Unfortunately, I didnot get a specific product. There might be problems elsewhere including the PCR conditions, primers and so on. But I want to make sure one thing first, is this promoter present in the genomic DNA of the cell line that I used? Although the gene is only expressed in specific tissues, I thought genomic DNA should be no big different among different tissues. That is why I didnt pay much attention to the template DNA and used the one that I have on hand. Am I wrong? Should I use the gene specific tissue for promoter amplification?

#2 bob1

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Posted 13 July 2012 - 07:09 PM

The promoter should be about the same in most cell lines from the same species, though there could be a number of mutations etc., that might have taken place, dependent on the cell line.

#3 gyma

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Posted 14 July 2012 - 06:39 AM

that means theoretically, I should be able to get the promoter sequence from the genomic DNA that I used, even if the gene is not expressed at all?

#4 bob1

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Posted 14 July 2012 - 04:31 PM

Yes.

#5 gyma

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Posted 16 July 2012 - 12:49 AM

Yes.

thank you very much.




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