Posted 12 July 2012 - 10:47 PM
Hi everyone, I tried to amplify a promoter region of my interested gene from genomic DNA of a cell line. Unfortunately, I didnot get a specific product. There might be problems elsewhere including the PCR conditions, primers and so on. But I want to make sure one thing first, is this promoter present in the genomic DNA of the cell line that I used? Although the gene is only expressed in specific tissues, I thought genomic DNA should be no big different among different tissues. That is why I didnt pay much attention to the template DNA and used the one that I have on hand. Am I wrong? Should I use the gene specific tissue for promoter amplification?