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can I use microplate reader for INF-gamma ELISPOT assay?

Started by joy123, Jul 12 2012 11:47 AM

ELISPOT IFN-gamma

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2 replies to this topic

#1 joy123

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Posted 12 July 2012 - 11:47 AM

Hi, I pan to do ELISPT assay to detect the level of IFN-gamma released by PBMC under stimulation of certain pathogens.

Now I have difficuly in finding an ELISPOT machine.

I wonder if it can be replaced by other 96-well plate reader? We have a reader in lab that suitable for fluorescence and other non fluorescence light, and the temperature can be settled.

I don't quite undersdand the difference ot ELISPOT machines compared to other microplate readers.

Would you please give me some suggestions? Thanks a lot!

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#2 bob1

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Posted 12 July 2012 - 12:55 PM

It sounds like you can, provided you have the appropriate filter(s) for your detection.

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#3 joy123

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Posted 12 July 2012 - 01:19 PM

bob1, on 12 July 2012 - 12:55 PM, said:

It sounds like you can, provided you have the appropriate filter(s) for your detection.

Thanks! I just understand that ELISPOT is counting spots formed in the well. I am not sure it can be read by microplate reader.

Besides, it supposed to be read mannually under microscope. I wonder if it needs special type of microscope, or it doesn't matter?