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ORAC assay - Hydrophobic Powder Dissolving Problems


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#1 mdarley

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Posted 12 July 2012 - 11:41 AM

Hey All,

I am running an ORAC (oxygen radical absorbance capacity) assay and I'm having some problems.

I have a hydrophobic powder that I want to test but it has to be in solution to do so... and it will not go into solution. I have tried Acetone, Methanol, and Chloroform with no luck. It falls out of solution almost immediately after mixing. I have attempted some heating and on a rocker plate for 1 hour. When the movement stops, it falls out and the heating didn't seem to change much.

Can anyone give me some tips on a better solvent to use or ORAC advice in general?

Thank you,

Meghan

#2 hobglobin

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Posted 12 July 2012 - 11:56 AM

Perhaps an oil and/or a surfactant then?
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 mdarley

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Posted 12 July 2012 - 12:31 PM

I could try that but I think don't think the reader would pick anything. The whole system is aquesous. Thank you though. I'm going to give it a try...

#4 bob1

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Posted 12 July 2012 - 12:53 PM

How about DMSO or DMF?




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