Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

serumm free culture of adherent cho cells

serumm free culture cho cells

  • Please log in to reply
1 reply to this topic

#1 sb21

sb21

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 11 July 2012 - 07:21 AM

Hi all,

i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media for 72-96h. i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions which medium i can use to sustain 72-96h time?

thnx

#2 Genewrangler

Genewrangler

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 02 August 2012 - 03:16 PM

I use Freestyle CHO media from Gibco, shake flasks at ~120rpm.





Also tagged with one or more of these keywords: serumm free culture, cho cells

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.