As far as SDS-PAGE gels, I would use a higher % gel for lower M.W. protein.
My boss asked me what percent gel I should use for Fab and F(ab')2.
I suggested 18% for Fab and 12% for F(ab')2 for a SDS-PAGE gel.
He says it is not quite correct.
I am thinking it is because he is looking to use a native urea gel [he asked when I would use a urea gel and if I knew what a native gel was right before he asked what % gel I would use for Fab and F(ab')2].
What % native urea gel should I use and why?
If you could link the Invitrogen gel cassette for the gel you are referring to, that will be helpful as well.
Edited by buzzking00, 09 July 2012 - 03:34 PM.