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transfecting siRNA thp-1 after differentiated to macrophage

siRNA thp-1 transfecting differentiated macrophage

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2 replies to this topic

#1 memari

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Posted 09 July 2012 - 12:43 PM

Do you know a simple way to transfect (siRNA) of thp-1 after differentiated to macrophage?
Lipofectamin did not work.
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Babak Memari

#2 Curtis

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Posted 15 July 2012 - 01:22 AM

it must work. what is your protocol?

#3 Vassil

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Posted 20 September 2012 - 10:47 AM

Hello memari,

When THP1s are differentiated into macrophages, they stop dividing. For Lipofectamine 2000 or Lipofectamine RNAiMAX you need the cells to be dividing in order for the siRNA to be introduced into the cell. You may try some protein-based transfection reagents.





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