Knockdown induce apoptosis
Posted 09 July 2012 - 06:22 AM
Posted 11 July 2012 - 12:29 AM
Does that make things more clear?
Posted 11 July 2012 - 07:54 PM
There are various proteins within the intrinsic and extrinsic pathways, and when you block one of them, other ones come in play. For example, I used to try kcocking down Bax, but still my cells died after infection (my virus induces apoptosis). Although the rate of death was slower compared to original cells, I could not reach a full stop. It was like other Bcl-2 proteins took part in absence of Bax. So I suggest to not play with those. Do not play with any Bcl-2 family of proteins. Even Bcl-2 itself, because Bcl-XL can almost do the same job, etc. the proteins of the extrinsic pathway are also the same.
So I agree with you to target proteins of the electron transport chain. But why not try structral proteins?
Posted 12 July 2012 - 04:10 AM
Structural proteins can of course be tried, do you have a suggestion on which one?
Posted 18 July 2012 - 09:51 PM