protien purification using flag affinity
Posted 05 July 2012 - 10:39 PM
This is to request you to kindly guide me through a problem .I have a flag tagged protein and in order to purify it I have anti flag resin to follow immunoprecipitation . Now I need to elute my protein from resin in its native form with no harm on my protein. Other options available and provided are adding Flag peptide or 3X peptide for competitive inhibition is geting to costly. Also adding in enterokinase would not help..Kindly guide me through this hurdle as early as possible.as to how could I elute/seperate my Flag tagged protien after immunoprecipitation..
Posted 06 July 2012 - 07:11 AM
Posted 08 July 2012 - 09:21 AM
Posted 09 December 2012 - 09:43 PM