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Strange Bands


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#1 WestKelvin

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Posted 04 July 2012 - 09:41 PM

Hi,

When I extracted my RNA from mouse liver I treated it with DNAse 1 from Ambion and I ran a gel after the extraction. It showed the normal RNA bands but also a band which is 10kb. I wonder is that genomics DNA?

Thanks!

#2 bob1

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Posted 05 July 2012 - 01:34 AM

No, genomic DNA will appear much larger than 10 kbp, and will appear as a smear not a band.

#3 xxl19901020

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Posted 05 July 2012 - 03:10 AM

Even to the bacteria, the genomics DNA is much larger than 10Kb, let alone the genomic DNA of animals




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