Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Help propogating Chlamydia stock


  • Please log in to reply
No replies to this topic

#1 nube

nube

    member

  • Active Members
  • Pip
  • 12 posts
0
Neutral

Posted 04 July 2012 - 03:13 PM

Hi All,

I am about to start trying to culture Chlamydia (C. pecorum and C. trachomatis, in BGM cells), and have never done any cell culture work before.

I have a few 100uL aliquots of C. trachomatis stock that I need to grow up in order to make 100's of aliquots, to use as my positive control. Each aliquot is apparently enough to inoculate one shell vial. Does anybody have a protocol for how to amplify the Chlamydia? Do I just grow up one of the aliquots in a shell vial, and after incubation use the supernatant to inoculate further shell vials, until I have a great enough volume? Or do I need to disrupt the cells somehow to release the EB's before harvesting the supernatant?

I'm sorry if this is a very basic question, but I am struggling to find a protocol for doing this. Any help will be greatly appreciated!

Thank you!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.