SDS precipitation after adding DNA binding buffer for column purificationIP DNA purification
Posted 04 July 2012 - 06:38 AM
I'm using Zymo columns for purifying IP'ed DNA after elution with SDS and NaHCO3. SDS precipitates and I wonder if it could affect the yield of the purification.
I've tried heating the samples after adding the binding buffer and before adding the mixture to the coluns, but I wonder if Qiagen kits would be better or if there's another way to elute mi IP from Protein A Sepharose to avoid this issue.
Posted 10 July 2012 - 02:07 PM
Instead of boiling, you can also place your chelex containing samples in a ThermoMixer set at 97 degrees with 1300rpm shaking
Edited by chabraha, 10 July 2012 - 02:09 PM.