I am having a small problem here...
My project is to extract and analyse some SNP (single nucleotide polymorphism) of two genes, namely BDNF (brain-derived neurotrophic factor) and ApoE (apolipoprotein E) of human subjects. I am kinda lost on the feasible (yet not too expensive) ways to do so. For BDNF, the whole gene is 247-AA long & 26kDa, located at 11p13; while it is 317-AA & 36kDa, located at 19q13.2 for ApoE. My samples are human whole blood samples.
Is it possible if i would like to selectively cut and sequence only the 2 specific genes via PCR, and send the whole gene for sequencing (since BDNF is only app. 750bp, and ApoE is only app. 950bp long)? What types of PCR should i use?
Thanks

Regards,
Tony.