At the precipitation step, I added absolute ethanol instead of isopropanol to my DNA elute and didn't even see a trace of pellet (which I was expecting to see) after centrifugation (spun at 15,000g 30min 4C).
Will the use of ethanol instead of isopropanol affect DNA precipitation??
Just another note, the Qiagen kit was dated 2009 - solution 1 and 3 were appropriately stored at 4C.
Edited by science noob, 03 July 2012 - 08:27 PM.