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Calcium Phosphate transfection - storage?


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#1 Grenouille

Grenouille

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Posted 03 July 2012 - 05:42 AM

Hi,

I just did something stupid and realized that s.th. went wrong with my cells AFTER I completely finished pipetting my calcium phosphate transfection mixes.
I don't want to throw it away :( !!!

Now I am wondering if it is possible to store my HeBS/CaCl2/DNA Mixes o/N @ 4°C and do the transfection tommorow.

Has anyone tried this?

So much DNA would be gone :(

Thanks in advance,

stupid me!

#2 LostintheLab

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Posted 03 July 2012 - 06:14 AM

Hi,

I think you should probably just make fresh tomorrow. Ca phosphate transductions are quite prone to temperature and pH changes that can really affect transfection efficiency, along with the precipitate fine-ness. Its not too much effort to make up the buffers fresh again (if you have the stocks of course) and its not expensive either.

Lost in the lab
I knew it! I knew it! Well, not in the sense of having the slightest idea, but I knew there was something I didn't know.




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