Primer for identification of yeast (ITS or 18Sr RNA gene)
Posted 03 July 2012 - 12:35 AM
I would like to identify yeast from fermented beverages and not sure should i use 18S rRNA gene or ITS or both? What is the primer sequence is good for the identification purposes?
Posted 03 July 2012 - 12:46 PM
Incidentally in most of the western world the yeast will be Saccharomyces cerevisiae (brewer's/baker's yeast).
Posted 03 July 2012 - 05:26 PM
The yeast i mean here is not restricted to Saccharomyces cerevisiae, it can be Candida, Pichia,
Edited by lactolee, 03 July 2012 - 05:27 PM.
Posted 04 July 2012 - 05:39 PM
I think you should probably do both, the 18s will give you identification down to genus level and then the ITS can be used further to identify the species and subtypes, if I recall my stuff about ITS regions correctly (inferring from plants here).