Koeng, on 03 July 2012 - 03:20 PM, said:
Yes I know what lacz is important for. (Lactose metabolism) I mean when I say "I just want ampicillin in the agar plates" because if I had the lacz gene, I would have to put X-gal in the solution to make it turn blue. And yes I meant bla promoter. I would just like some input, like "you should do this or that", to continue this fun project. (well, fun for me). Maybe some nice MCS sites from different plasmids, if you remember that plasmid just say it and post I can look it up. Or maybe a different resistance, because that resistance gene is better or shorted or because personally when you where in high school the ampiciilin in your plates didn't work but Kanamycin worked really well.
Also thanks for the idea of some new programs, I am looking into them and will probably spend the rest of the night making a really nice map. But right now I'll just paste the pdf of the map I make with ApE.
I see what you are trying to do, however: there isnt a lot of "use" when you do it like you do it.
I know that you are trying to improve the plamid, but the problem is that you dont have a "goal", I mean: you can indeed make the plasmid smaller, but whats the point of there is no goal or meaning.
Maybe you should try to look into more practical things like: how can I adjust a certain plasmid to make it a plasmid that I can use for both gram + and gram - bacteria?
Or can I make a plasmid that can be used as a indicator for a certain transformation (eg: how can I show that the insert in indeed present in the plasmid?)
Or like you allready mentioned: what antibiotic gene should be used and why? For example: gram + against gram -: is this the same antibiotic? Or what with antibiotics that are light sensitive...? What about the price of the antibiotics? WHat about the "safety" of the antibiotics...
I know this is a lot of information, but if you have some time and you think its fun, you could try to figure out what "parts" of a plasmid are important and what possibilities are out there.
(eg: what do you need: promoters, markers, MCS, origins.....)
You see what I mean?
Edited by pito, 04 July 2012 - 02:29 AM.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.