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How to precipitate RNA from water

rna precipitation

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3 replies to this topic

#1 graceahne

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Posted 02 July 2012 - 02:15 PM

I used the trizol protocol to isolate RNA and have dissolved the RNA in too much water to accurately quantify. How do I precipitate the RNA again from the water?

#2 Curtis

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Posted 02 July 2012 - 10:36 PM

How much water is that? you can add pure iso-p as much as you can, then centrifuge for 15 min at least, at high speed. Additional iso-p won't harm your RNA.

#3 rmbio

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Posted 09 July 2012 - 11:11 PM

yes, you can precipitate the diluted RNA with equal volume of isopropanol, incubate at -20 for 15-30 min, pellete down by centrifuging at 10000 g/4C/10 min and dissolve the pellet in the desired volume of water

#4 mdfenko

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Posted 11 July 2012 - 06:01 AM

don't forget to add salt
talent does what it can
genius does what it must
i do what i get paid to do





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