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N acetyl cysteine solution for cell culture

NAC

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#1 12345678

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Posted 02 July 2012 - 09:40 AM

Hi! I need to dissolve N acetyl cysteine into cell culture media. I need it at 25 mM which is 40mg/mL.

How do I do this with minimal interference in ionic strength?

Or can i buy a stock solution of it, that is already at physiological pH?

Thanks.

#2 Curtis

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Posted 03 July 2012 - 12:24 AM

Is it really difficult to dissolve? Posted Image

#3 PHVS

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Posted 03 July 2012 - 06:39 PM

If you got yours from Sigma it is soluble at 100 mg/ml in H2O (heating). I've used it some times and the medium pH drops a bit.

#4 Dennis_Simpson

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Posted 04 July 2012 - 06:42 AM

NAC is really easy to dissolve. You are using it at a fairly high concentration so you could dissolve it in your growth media. Did you mean to write mM and not uM for your working concentration? When I have used this in the past I found that mM concentrations kill cells.




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