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Transformation of an inducible GFP gene into Aspergillus niger

GFP Aspergillus niger transformation

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#1 Ruth Grace

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Posted 01 July 2012 - 06:09 PM

Hello, I am doing the transformation of an inducible GFP gene into Aspergillus niger for my final year project. I am BSc student and looking for opinion on the best method to transform the GFP in A.niger (besides using Agrobacterium). Also, I would like to ask opinion on how to screen the transformed fungus. Thank you. Posted Image

#2 bob1

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Posted 02 July 2012 - 01:14 PM

I don;t know about transformation of ungi, but I would probably invesitgate electroporation.

You should be able to screen by any one of the basic molecular methods such as PCR, western blotting, immuo-fluorescence or even if the cells are induced plain old GFP fluorescence.

#3 Ruth Grace

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Posted 03 July 2012 - 05:35 PM

Thank you! :D





Also tagged with one or more of these keywords: GFP, Aspergillus niger, transformation

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