2 replies to this topic

[Ruth Grace]

Hello, I am doing the transformation of an inducible GFP gene into Aspergillus niger for my final year project. I am BSc student and looking for opinion on the best method to transform the GFP in A.niger (besides using Agrobacterium). Also, I would like to ask opinion on how to screen the transformed fungus. Thank you.

[bob1]

I don;t know about transformation of ungi, but I would probably invesitgate electroporation.

You should be able to screen by any one of the basic molecular methods such as PCR, western blotting, immuo-fluorescence or even if the cells are induced plain old GFP fluorescence.

[Ruth Grace]

Thank you!