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Hybridoma development


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#1 amarnathbt

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Posted 30 June 2012 - 01:02 AM

Hi all,
Very recently I am observing that fusion plates are forming clumps ( 2nd/3rd day) and hybridomas started dieng.... I tried changing all possible reagents and even new vial of Sp2/O has been thawed freshly for fusion though the result is same, cell started clumping, but the control wells are fine (where only spleen and only Sp2/O plated in HAT). Spleen is from immunized Balb/C mice... Can any one please suggest .....

#2 Deborah

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Posted 27 August 2013 - 03:41 AM

Hi, probably it's too late anyway...did you check mycoplasma contamination? This is one of the most common reasons of no hybridoma development






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