Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Inorganic phosphorous

  • Please log in to reply
1 reply to this topic

#1 InduS



  • Active Members
  • Pip
  • 21 posts

Posted 30 June 2012 - 12:25 AM

I'm trying out for the quantification of inorganic phosphorus in my samples. I've tried it out with Fiske Subbarow and Munoz et al methods.....But i'm finding it difficult to get consistence reading in both the methods. I know there are number of factors that could have went wrong but I'm zeroing in on improper cleaning of my Test tubes..... can anyone tell me what the best method is to clean the test tubes off inorganic phosphates? Will acid -water do the trick? or any thing else?


#2 mdfenko


    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,811 posts

Posted 02 July 2012 - 07:25 AM

we washed with phosphate-free detergent (not really phosphate-free when using sensitive phosphate assay) and rinsed with copious amounts of deionized water.

we measured inorganic phosphate using a method modified from chen, et al, anal. chem., 28: 1756-58 (1956). it is more sensitive than fiske-subbarow.
talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.