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Normalize to cDNA for rtPCR?


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#1 dolphin12



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Posted 29 June 2012 - 09:32 PM

When I do rtPCRs I usually normalize to RNA prior to reverse transcription. However, due to an error one of my recent set of samples was not properly normalized to RNA concentrations and now I ended up with cDNAs at varying concentrations spanning the 800-1000ng/ul. Unfortunately, there is no more RNA left from this set of samples for me to repeat the reverse transcription for. Is there anyway I can salvage the cDNA samples by using the nanodrop to detect their concentration and normalizing to cDNA instead? Would this work? Thanks for any information.

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